中国水稻科学 ›› 2012, Vol. 26 ›› Issue (3): 351-355.DOI: 10.3969/j.issn.10017216.2012.03.014

• 研究简报 • 上一篇    下一篇

两个环境下糙米和精米蛋白质含量的QTL分析

杨亚春,倪大虎,宋丰顺,李莉,冯光,李泽福,杨剑波*   

  1. 安徽省农业科学院 水稻研究所, 安徽 合肥  230031;
  • 收稿日期:2011-09-13 修回日期:2011-10-19 出版日期:2012-05-10 发布日期:2012-05-10
  • 通讯作者: 杨剑波*
  • 基金资助:

    国家自然科学基金资助项目(31071401);科技部转基因重大专项(2008ZX08001002, 2009ZX08001013B);安徽省优秀青年基金资助项目(10040606Y03); 国家863计划资助项目(2012AA1011024)。

Identification of QTL for Protein Content in Brown and Milled Rice in Two Enironments

YANG  Yachun, NI  Dahu, SONG  Fengshun, LI  Li, FENG  Guang,  LI  Zefu, YANG  Jianbo*   

  1. Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China;
  • Received:2011-09-13 Revised:2011-10-19 Online:2012-05-10 Published:2012-05-10
  • Contact: YANG Jianbo*

摘要: 利用两个直链淀粉含量相似的水稻品种9311和日本晴为亲本,采取单粒传法创建由190个家系组成的重组自交系群体,并构建了包含202个SSR、CAP和STS标记的遗传连锁图谱。采用复合区间作图法,在两个不同生态环境下(三亚、合肥)对糙米蛋白质含量和精米蛋白质含量的QTL进行了定位分析,共定位到9个QTL。其中,qBRPC1.3在两个环境中均被检测到,稳定性较高,其贡献率达9.4%;位于第1染色体的RM578-RM10596是多效性区间,同时控制糙米蛋白质含量和精米蛋白质含量,贡献率分别为6.4%和5.7%。

关键词: 水稻, 蛋白质含量, 数量性状基因座

Abstract: Based on single seed descend method a recombinant inbred lines  population consisting of 190 lines  was created by crossing two rice varieties with similar apparent amylose content, Nipponbare (japonica) and 9311 (indica) by QTLs for brown rice protein content(BRPC) and milled rice protein content (MRPC) with a genetic linkage map including 202 SSR, CAP and STS markers were identified. By composite interval mapping method in two environments sites (Sanya Hainan Province, China and Hefei Anhui Province, China), a total of nine QTLs were identified, indicating that rice protein content was controlled by multiple genes.  qBRPC1.3 was detected at the two sites with high stability, explaining 9.4% of total variation. The interval RM578-RM10596 on chromosome 1, controlled BRPC and MRPC, explaining 6.4% and 5.7% of total variation, respectively. 

Key words: rice, protein content, quantitative trait locus

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